1.0Novaptechhttps://novaptech.com/enrich600338<blockquote class="wp-embedded-content" data-secret="u9j14t68aq"><a href="https://novaptech.com/en/second-generation-aptamers-selkiss-aptaswitch/">Novaptech structure switching aptamers</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://novaptech.com/en/second-generation-aptamers-selkiss-aptaswitch/embed/#?secret=u9j14t68aq" width="600" height="338" title="“Novaptech structure switching aptamers” — Novaptech" data-secret="u9j14t68aq" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script> /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); //# sourceURL=https://novaptech.com/wp-includes/js/wp-embed.min.js </script> Structure-switching aptamers Aptaswich Aptamers are known to generally undergo a conformational change upon binding to their ligand. This can be exploited for their selection and for signaling the presence of the target. The structural change is then associated to a signal (fluorescence energy transfer for instance). This can be deliberately exploited for the design of aptamer-based biosensors. Tell us about your assay The NOVAswitch technology The selection is carried out against free molecules in solution whereas the oligonucleotide library is immobilized in a support. Candidates released in the solution following the addition of the target molecule are collected, amplified and analyzed. In another format the selection takes advantage of so-called kissing interactions. In this case, the addition of the target promotes the interaction of candidates to a hairpin oligonucleotide that are subsequently collected, amplified and analyzed. The selected candidates are functional aptaswitch. They can be integrated into biosensors with different modalities of signal transduction. Examples of application Durand, G., Lisi, S., Ravelet, C., Dausse, E., Peyrin, E., & Toulmé, J.-J. (2014). Riboswitches Based on Kissing Complexes for the Detection of Small Ligands. Angewandte Chemie International Edition, 53(24), 6105–6110. Durand, G., Dausse, E., Goux, E., Fiore, E., Peyrin, E., Ravelet, C., & Toulmé, J.-J. (2016). A combinatorial approach to the repertoire of RNA kissing motifs; towards multiplex detection by switching hairpin aptamers. Nucleic Acids Research, 44(9), 4450-4459. Goux, E., Dausse, E., Guieu, V., Azéma, L., Durand, G., Henry, M., Choisnard, L., Toulmé, J.-J., Raveleta, C., & Peyrin, E. (2017). A colorimetric nanosensor based on a selective target-responsive aptamer kissing complex. Nanoscale, Issue 12. Learn about our R&D program for rapid & multiplex testing Aptaswitch-based assays Rapid tests Aptamer lateral flow assays Environment & food safety monitoring Detection of a drug in biological fluids They support our R&D Any question ? Just ask us ! Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulmé, PhD, CSOhttps://novaptech.com/wp-content/uploads/2024/06/thumb_600_0_0_0_auto.jpg