{"id":1637,"date":"2024-06-11T13:41:04","date_gmt":"2024-06-11T13:41:04","guid":{"rendered":"https:\/\/novaptech.com\/?page_id=1637"},"modified":"2025-02-28T13:13:18","modified_gmt":"2025-02-28T13:13:18","slug":"biomarker-discovery","status":"publish","type":"page","link":"https:\/\/novaptech.com\/en\/biomarker-discovery\/","title":{"rendered":"Biomarker discovery | Novaptech"},"content":{"rendered":"\t\t
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The role of aptamers in biomarker visualization and analysis<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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In the advanced medical sector, biomarker research and detection are key factors in early diagnosis, monitoring disease progression and personalizing treatment. <\/span>
Biomarkers are measurable biological indicators that can be found in blood, urine, tissue or other body fluids, providing crucial information about an individual’s state of health. <\/span>
These molecules, whether proteins, nucleic acids or other substances, enable doctors and researchers to detect, diagnose and monitor a variety of diseases, including cancer, heart disease, diabetes and many others. <\/span>
Aptamers are DNA or RNA molecules selected in the laboratory to bind specifically to biological targets. This technology opens up new perspectives in biomarker analysis. Their ability to precisely target specific molecules and their flexibility oh synthesis make them the tools of choice for the detection and identification of biomarkers.<\/span><\/p>

Learn more about the revolutionary aptamer technology and its selection process through aptamer SELEX. Understand the benefits of aptamers vs antibodies and explore comprehensive methods of biomarker analysis. Dive into the advanced research conducted in our biotechnology laboratory.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Key advantages of Aptamers over Antibodies<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Aptamers are synthetic molecules that can be raised against any kind of target, including toxic or non-immunogenic ones. They bind their target with affinity similar or higher than antibodies. They are 10 fold smaller than antibodies and can be chemically-modified at will in a defined and precise way. They can be easily stored and delivered ; they can be reversibly heat-denatured and renatured. The sourcing is guaranteed with a total batch to batch reproducibility.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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They can be used under non physiological conditions for analytical purposes.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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They are non\u2013immunogenic and can be used in vivo.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\"Aptamers<\/figure><\/div>\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tNovaptech's aptamer services<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Any question ? Contact us<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days).<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tContact us<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Your scientific contact :<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Jean-Jacques Toulm\u00e9, PhD, CSO<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tAnalysis of biomarkers via aptamers<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Aptamers are nucleic acid molecules, either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid), which are used in medicine, biomedical research and analytical biochemistry and chemistry.
They are distinguished by their ability to bind selectively and strongly to specific targets, be they proteins, small chemical compounds or even cells.<\/p>

Aptamer design begins in the Novaptech laboratory, where researchers use a process called SELEX (Systematic Evolution of Ligands by EXponential enrichment) to isolate aptamers with a particularly high affinity for the desired target. This is a complex, multi-step process:<\/p>

1. In vitro selection<\/strong> : The selection process begins with a random library of oligonucleotides (DNA or RNA oligomers) containing billions of different sequences. These sequences are exposed to the target one wishes to detect or target. The aptamers that bind to the target are then recovered.<\/p>

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2. Amplification<\/strong> : The sequences that bound to the target are amplified by PCR (polymerase chain reaction) to produce a larger quantity of candidates.<\/p>


3. Repeating the process<\/strong> : This selection cycle is repeated several times to improve the affinity and specificity of the candidates towards the target.<\/p>

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4. Validation<\/strong> : The final candidates obtained are then tested to confirm their ability to bind specifically to the target. Oligonucleotides that meet the specificity and affinity criteria are aptamers.<\/p>

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This in vitro selection process enables researchers at Novaptech to generate tailor-made aptamers for virtually any target. Once obtained, aptamers can be used in a variety of ways, including the detection of the target in various samples, inhibition of the activity of specific
proteins or even targeted drug delivery.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tHow are aptamers revolutionizing biomarker analysis ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Visualization of known biomarkers using aptamers<\/strong><\/p>

\u00b7 Production of labeled aptamers : Selected aptamers are labeled with fluorescent molecules, enzymes or nanoparticles to facilitate their subsequent detection.
\u00b7 Interaction with the target : When exposed to a biological sample containing the desired target, aptamers bind specifically to that target.
\u00b7 Detection and quantification : Aptamers bound to the target are then detected and quantified. This can be done using a variety of methods, such as colorimetry, fluorescence, electrochemistry or PCR, depending on how the aptamers were labeled.
1.
\u00b7 Oligonucleotide aptamers selected for their high binding affinity to specific targets, are revolutionizing the field of biomolecular detection, thanks to their flexibility, specificity and ease of modification.
2.
\u00b7 They offer a validated alternative to antibodies in a variety of analytical applications, notably in the development of biosensors.
3.
\u00b7 The specificity of aptamers derives from their ability to fold into three-dimensional structures enabling strong, specific binding to their target, whether small molecules, proteins or even whole cells.
4.
\u00b7 This specificity is essential for detection and analytical applications, where distinguishing between similar molecules provides precise diagnosis.
5.
\u00b7 The versatility of aptamers is also a major asset. Thanks to chemical modifications, it is possible to improve their stability under physiological conditions, increase their in vivo half-life or endow them with functional groups facilitating their immobilization on sensor surfaces or labeling with fluorescent or electrochemical probes.
6.
\u00b7 The development of aptamer-based biosensors benefits directly from these features. Aptamers can be integrated into detection systems that translate molecular recognition into a measurable electrical or optical signal.
7.
\u00b7 This signal transduction enables precise quantification of the target in complex samples, paving the way for rapid, sensitive and inexpensive diagnostics for a wide range of applications, from environmental monitoring to early disease diagnostic.
8.
\u00b7 The future of aptamers in biosensors is very promising, and through its research and development process, Novaptech aims to further improve the sensitivity and specificity of detection systems.
9.
\u00b7 The engineering of aptamers for targeted applications could revolutionize the diagnosis and treatment of diseases, as well as the detection of analytes further affirming their indispensable role in the biomedical field.
10.<\/p>

Advantages of aptamers over other detection tools<\/strong><\/p>

Aptamers offer several significant advantages over other biomarker visualization and analytical tools
\u00b7 High selectivity : Aptamers are designed to bind extremely specifically to their target, minimizing false positives and interference.
\u00b7 Ease of production : Aptamers can be produced in large quantities, economically, relatively simply and reproducibly.
\u00b7 Stability : Unlike proteins, aptamers are thermally stable, making them ideal for use in a variety of environments.
\u00b7 Versatility : Aptamers can be used to detect a wide range of biomarkers, from proteins to small molecules to cells.<\/p>

Examples of successful biomarker visualization using aptamers<\/strong><\/p>

\u00b7 Detection of cancer proteins : Aptamers have been developed to detect specific proteins associated with cancer, such as epidermal growth factor (EGF) in breast cancer. These aptamers enable early detection of the disease.
\u00b7 Infectious disease monitoring : Aptamers have been used to detect pathogens such as HIV, dengue fever virus and SARS-CoV-2 (responsible for COVID-19), enabling rapid and accurate diagnosis, notably through the use of rapid test kits and devices.
\u00b7 Detection of cardiac biomarkers : Aptamers have been developed to detect cardiac biomarkers such as troponin, which is essential for diagnosing heart problems.
Aptamers represent a significant advance in biomarker detection and analysis in medicine. Their high specificity, versatility and ease of production make them powerful tools for early disease detection, personalized treatment and biomedical research.
Thanks to their capacity and Novaptech’s expertise, aptamers represent a major advance in the visualization and analysis of biomarkers in medicine.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

The role of aptamers in biomarker visualization and analysis In the advanced medical sector, biomarker research and detection are key factors in early diagnosis, monitoring disease progression and personalizing treatment. Biomarkers are measurable biological indicators that can be found in blood, urine, tissue or other body fluids, providing crucial information about an individual’s state of health. These molecules, whether proteins, nucleic acids or other substances, enable doctors and researchers to detect, diagnose and monitor a variety of diseases, including cancer, heart disease, diabetes and many others. Aptamers are DNA or RNA molecules selected in the laboratory to bind specifically to biological targets. This technology opens up new perspectives in biomarker analysis. Their ability to precisely target specific molecules and their flexibility oh synthesis make them the tools of choice for the detection and identification of biomarkers. Learn more about the revolutionary aptamer technology and its selection process through aptamer SELEX. Understand the benefits of aptamers vs antibodies and explore comprehensive methods of biomarker analysis. Dive into the advanced research conducted in our biotechnology laboratory. Key advantages of Aptamers over Antibodies Aptamers are synthetic molecules that can be raised against any kind of target, including toxic or non-immunogenic ones. They bind their target with affinity similar or higher than antibodies. They are 10 fold smaller than antibodies and can be chemically-modified at will in a defined and precise way. They can be easily stored and delivered ; they can be reversibly heat-denatured and renatured. The sourcing is guaranteed with a total batch to batch reproducibility. They can be used under non physiological conditions for analytical purposes. They are non\u2013immunogenic and can be used in vivo. Novaptech’s aptamer services Any question ? Contact us Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulm\u00e9, PhD, CSO Analysis of biomarkers via aptamers Aptamers are nucleic acid molecules, either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid), which are used in medicine, biomedical research and analytical biochemistry and chemistry. They are distinguished by their ability to bind selectively and strongly to specific targets, be they proteins, small chemical compounds or even cells. Aptamer design begins in the Novaptech laboratory, where researchers use a process called SELEX (Systematic Evolution of Ligands by EXponential enrichment) to isolate aptamers with a particularly high affinity for the desired target. This is a complex, multi-step process: 1. In vitro selection : The selection process begins with a random library of oligonucleotides (DNA or RNA oligomers) containing billions of different sequences. These sequences are exposed to the target one wishes to detect or target. The aptamers that bind to the target are then recovered. \u00a0 2. Amplification : The sequences that bound to the target are amplified by PCR (polymerase chain reaction) to produce a larger quantity of candidates. 3. Repeating the process : This selection cycle is repeated several times to improve the affinity and specificity of the candidates towards the target. \u00a0 4. Validation : The final candidates obtained are then tested to confirm their ability to bind specifically to the target. Oligonucleotides that meet the specificity and affinity criteria are aptamers. \u00a0 This in vitro selection process enables researchers at Novaptech to generate tailor-made aptamers for virtually any target. Once obtained, aptamers can be used in a variety of ways, including the detection of the target in various samples, inhibition of the activity of specific proteins or even targeted drug delivery. How are aptamers revolutionizing biomarker analysis ? Visualization of known biomarkers using aptamers \u00b7 Production of labeled aptamers : Selected aptamers are labeled with fluorescent molecules, enzymes or nanoparticles to facilitate their subsequent detection. \u00b7 Interaction with the target : When exposed to a biological sample containing the desired target, aptamers bind specifically to that target. \u00b7 Detection and quantification : Aptamers bound to the target are then detected and quantified. This can be done using a variety of methods, such as colorimetry, fluorescence, electrochemistry or PCR, depending on how the aptamers were labeled. 1.\u00b7 Oligonucleotide aptamers selected for their high binding affinity to specific targets, are revolutionizing the field of biomolecular detection, thanks to their flexibility, specificity and ease of modification. 2.\u00b7 They offer a validated alternative to antibodies in a variety of analytical applications, notably in the development of biosensors. 3.\u00b7 The specificity of aptamers derives from their ability to fold into three-dimensional structures enabling strong, specific binding to their target, whether small molecules, proteins or even whole cells. 4.\u00b7 This specificity is essential for detection and analytical applications, where distinguishing between similar molecules provides precise diagnosis. 5.\u00b7 The versatility of aptamers is also a major asset. Thanks to chemical modifications, it is possible to improve their stability under physiological conditions, increase their in vivo half-life or endow them with functional groups facilitating their immobilization on sensor surfaces or labeling with fluorescent or electrochemical probes. 6.\u00b7 The development of aptamer-based biosensors benefits directly from these features. Aptamers can be integrated into detection systems that translate molecular recognition into a measurable electrical or optical signal. 7.\u00b7 This signal transduction enables precise quantification of the target in complex samples, paving the way for rapid, sensitive and inexpensive diagnostics for a wide range of applications, from environmental monitoring to early disease diagnostic. 8.\u00b7 The future of aptamers in biosensors is very promising, and through its research and development process, Novaptech aims to further improve the sensitivity and specificity of detection systems. 9.\u00b7 The engineering of aptamers for targeted applications could revolutionize the diagnosis and treatment of diseases, as well as the detection of analytes further affirming their indispensable role in the biomedical field.10. Advantages of aptamers over other detection tools Aptamers offer several significant advantages over other biomarker visualization and analytical tools \u00b7 High selectivity<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-1637","page","type-page","status-publish","hentry"],"yoast_head":"\nBiomarker discovery | Novaptech - Novaptech<\/title>\n<meta name=\"description\" content=\"In the advanced medical sector, biomarker research and detection are key factors in early diagnosis, monitoring disease progression and personalizing treatment.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/novaptech.com\/en\/biomarker-discovery\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biomarker discovery | Novaptech - 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