{"id":1650,"date":"2024-06-11T13:54:53","date_gmt":"2024-06-11T13:54:53","guid":{"rendered":"https:\/\/novaptech.com\/?page_id=1650"},"modified":"2025-02-28T13:22:31","modified_gmt":"2025-02-28T13:22:31","slug":"definition-of-an-aptamer-novaptech","status":"publish","type":"page","link":"https:\/\/novaptech.com\/en\/definition-of-an-aptamer-novaptech\/","title":{"rendered":"Definition of an aptamer | Novaptech"},"content":{"rendered":"\t\t
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What is an aptamer ? <\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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In the field of biotechnology, aptamers are attracting growing interest as they play a fundamental role in research and medicine. <\/span>These short synthetic DNA or RNA oligonucleotides are designed to bind specifically to a given target.\u00a0<\/span>Their interest lies in their ability to replace traditional antibodies and other affinity ligands.\u00a0<\/span>This power offers significant advantages in terms of cost, stability and specificity.\u00a0<\/span>Aptamers are small sequences of nucleic acid, DNA or RNA, selected in vitro for their ability to bind with high affinity to a specific target.\u00a0<\/span><\/p>

In the Novaptech laboratory, aptamers are identified using a technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment ) and can be adapted to recognize virtually any cell or molecule, be it protein, enzyme, drug or chemical compound.<\/span><\/p>

Discover the innovative Aptamer<\/a> technology and its applications in various fields of biotechnology.<\/span><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Key advantages of Aptamers over Antibodies<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Aptamers are synthetic molecules that can be raised against any kind of target, including toxic or non-immunogenic ones. They bind their target with affinity similar or higher than antibodies. They are 10 fold smaller than antibodies and can be chemically-modified at will in a defined and precise way. They can be easily stored and delivered ; they can be reversibly heat-denatured and renatured. The sourcing is guaranteed with a total batch to batch reproducibility.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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They can be used under non physiological conditions for analytical purposes.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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They are non\u2013immunogenic and can be used in vivo.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\"Aptamers<\/figure><\/div>\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tNovaptech's aptamer services<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Any question ? Contact us<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days).<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tContact us<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Your scientific contact :<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Jean-Jacques Toulm\u00e9, PhD, CSO<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tThe importance of aptamers in research and medicine<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

The selective binding capacity of aptamers has major implications in research and medicine.
They are used in a variety of fields, from the specific detection of biomarkers to the design of targeted drugs.
Aptamers are also of interest in analytical chemistry\/biochemistry for the quantitative detection of biomarkers or contaminants.<\/span><\/span><\/p>

Origin and discovery of aptamers<\/strong><\/span><\/p>

The history of aptamers dates back to the 1990s, when researchers began exploring new alternatives to traditional antibodies for the recognition of targets. <\/span>
The discovery and development of aptamers has been hailed as a major breakthrough in biotechnology. Jack Szostack and Larry Gold were pioneers in this field, and in particular helped define the foundations of the SELEX technology.<\/span><\/p>

Comparison with other probes and ligands<\/strong><\/span><\/p>

Compared to other ligands such as antibodies, aptamers offer several advantages. They are more stable, less costly to produce in large quantities, and their in vitro selection process is faster and more versatile. <\/span>
Aptamers can be developed to target specific molecules without the need for animal breeding or cell culture, making them particularly attractive for applications in research and medicine.<\/span><\/p>

How do aptamers work ?<\/strong><\/span><\/p>

The aptamer selection process is the cornerstone of the creation of specific aptamers. It begins with the generation of an initial library of random oligonucleotides. This library contains a multitude of DNA or RNA sequences, each with a unique three-dimensional structure. <\/span>
This library is then subjected to selection cycles in which aptamers with a high affinity for the desired target are isolated. These cycles consist of a series of steps that include binding to the target, washing to remove unbound sequences, and finally, elution of target-bound aptamers. <\/span>
This process is repeated several times iteratively until the aptamers with high specificity and affinity are selected by Novaptech.<\/span><\/p>

The specific binding mechanism of aptamers to their target<\/strong><\/span><\/p>

Aptamers use a variety of molecular interactions to bind to their target hydrogen bonds, electrostatic or\/and stacking interactions, between the aptamer nucleotides and the target residues. This interaction is highly specific, depending on the precise molecular characteristics of the target. <\/span>
Moreover, the aptamer three-dimensional conformation is essential for its specificity. The aptamer adopts a particular folded structure that perfectly matches the shape of the target. This ensures tight, specific binding.<\/span><\/p>

Examples of targets commonly studied with aptamers<\/strong><\/span><\/p>

Aptamers have become the tools of choice for the recognition and study of a variety of biomolecular targets. Commonly studied examples include proteins, peptides, viruses, bacteria, cells, drugs and chemical compounds. <\/span>
For example, aptamers have been developed to specifically target proteins such as thrombin, EGFR (epidermal growth factor receptor) and vascular endothelial growth factor (VEGF). <\/span>
These aptamers are used in biomedical research for applications ranging from biomarker to drug analysis.<\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tWhat are the applications of aptamers in research ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Use of aptamers in molecular biology<\/strong><\/p>

Today, aptamers are essential tools in molecular biology for studying various cellular and molecular processes.
They can be used to analyze the structure and function of proteins by binding specifically to them. For example, aptamers are used to study protein-protein interactions, to target specific regions of proteins or to selectively inhibit their activity.
These applications are essential for understanding the biological mechanisms underlying many diseases and for developing new therapies.<\/p>

Applications in diagnostics and detection<\/strong><\/p>

Thanks to their high specificity, aptamers are able to detect specific biomarkers associated with diseases, such as proteins or metabolites.
This capability enables Novaptech to create accurate, rapid and sensitive diagnostic tests for diseases such as cancer, viral infections or neurodegenerative diseases.
Aptamers are also used to detect toxic substances or contaminants in the environment (pesticides, endocrine disruptors, drug residues\u2026).<\/p>

Aptamers in pharmacology<\/strong><\/p>

In the pharmaceutical industry, aptamers are playing an increasingly important role in drug discovery. They can be designed to specifically target proteins involved in disease, paving the way for the development of more specific and less toxic drugs.
For example, aptamers are being studied to target cell receptors involved in cancer or autoimmune diseases.\u00a0<\/p>

In addition, aptamers can be used to deliver drugs directly to target cells, thus minimizing undesirable side effects.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tWhat are the applications of aptamers in medicine ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Detection of small molecules in medicine and environment<\/strong><\/p>

– Companion test
– Point of care or in the field analysis
– Food safety
– Pollution analysis<\/p>

Diagnostic applications<\/strong><\/p>

– Metabolite monitoring
– Biomarker detection<\/p>

Therapy with aptamers<\/strong><\/p>

Aptamers and Novaptech are revolutionizing the field of medicine by offering the possibility
of developing targeted therapies.
This approach enables drugs to be delivered specifically to diseased cells or tissues, thereby
reducing the risk of adverse effects on healthy tissue. Aptamers can be designed to target
specific receptors on the surface of cancer cells, virus-infected cells or cells involved in
autoimmune diseases.
This high-precision drug delivery enhances treatment efficacy while reducing side effects.<\/p>

Aptamers in cancer treatment<\/strong><\/p>

One of the applications of aptamers in medicine is in cancer treatment.
In this context, aptamers are designed to specifically target proteins overexpressed on the
surface of cancer cells, making them precise binding agents for the delivery of anti-cancer
drugs.
In addition, aptamers are used to block cancer cell growth signals or inhibit tumor
vascularization, representing a brake on cancer progression.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tWhat are the advantages of aptamers ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

The advantages of aptamers are numerous, reinforcing their position as an innovative solution in the development of new therapies, innovative diagnostic probes and robust analytical tools :
1. High binding specificity : aptamers are designed to bind highly specifically to their target, be it a protein, a virus or a small molecule. This specificity ensures that the aptamer interacts only with the intended target, minimizing undesirable side effects. This precision and affinity in binding is essential for the creation of tools in many applications.
2. Modifiability to improve their binding properties and function : Aptamers can be chemically modified to improve their stability in the biological environment, for their detection through their conjugation to a dye or for their grafting on a support.
3. Less immunogenic than antibodies : Unlike antibodies, are not or poorly immunogenic, less likely to trigger an immune response in the patient’s body.
This characteristic is particularly important during repeated use of a drug, as an undesirable immune response is likely to lead to a reduction in treatment efficacy.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tWhat are the future prospects for aptamers ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Although aptamers have already demonstrated their potential in numerous applications, challenges remain in the field of aptamer research.
Novaptech researchers are actively working on chemical modifications to improve their properties (affinity, lifetime\u2026).
Another challenge facing Novaptech teams is to develop even more efficient large-scale screening methods. Efforts are underway to automate and optimize the SELEX selection process to make our aptamer identification more efficient and cost-effective.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

What is an aptamer ? In the field of biotechnology, aptamers are attracting growing interest as they play a fundamental role in research and medicine. These short synthetic DNA or RNA oligonucleotides are designed to bind specifically to a given target.\u00a0Their interest lies in their ability to replace traditional antibodies and other affinity ligands.\u00a0This power offers significant advantages in terms of cost, stability and specificity.\u00a0Aptamers are small sequences of nucleic acid, DNA or RNA, selected in vitro for their ability to bind with high affinity to a specific target.\u00a0 In the Novaptech laboratory, aptamers are identified using a technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment ) and can be adapted to recognize virtually any cell or molecule, be it protein, enzyme, drug or chemical compound. Discover the innovative Aptamer technology and its applications in various fields of biotechnology. Key advantages of Aptamers over Antibodies Aptamers are synthetic molecules that can be raised against any kind of target, including toxic or non-immunogenic ones. They bind their target with affinity similar or higher than antibodies. They are 10 fold smaller than antibodies and can be chemically-modified at will in a defined and precise way. They can be easily stored and delivered ; they can be reversibly heat-denatured and renatured. The sourcing is guaranteed with a total batch to batch reproducibility. They can be used under non physiological conditions for analytical purposes. They are non\u2013immunogenic and can be used in vivo. Novaptech’s aptamer services Any question ? Contact us Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulm\u00e9, PhD, CSO The importance of aptamers in research and medicine The selective binding capacity of aptamers has major implications in research and medicine.They are used in a variety of fields, from the specific detection of biomarkers to the design of targeted drugs.Aptamers are also of interest in analytical chemistry\/biochemistry for the quantitative detection of biomarkers or contaminants. Origin and discovery of aptamers The history of aptamers dates back to the 1990s, when researchers began exploring new alternatives to traditional antibodies for the recognition of targets. The discovery and development of aptamers has been hailed as a major breakthrough in biotechnology. Jack Szostack and Larry Gold were pioneers in this field, and in particular helped define the foundations of the SELEX technology. Comparison with other probes and ligands Compared to other ligands such as antibodies, aptamers offer several advantages. They are more stable, less costly to produce in large quantities, and their in vitro selection process is faster and more versatile. Aptamers can be developed to target specific molecules without the need for animal breeding or cell culture, making them particularly attractive for applications in research and medicine. How do aptamers work ? The aptamer selection process is the cornerstone of the creation of specific aptamers. It begins with the generation of an initial library of random oligonucleotides. This library contains a multitude of DNA or RNA sequences, each with a unique three-dimensional structure. This library is then subjected to selection cycles in which aptamers with a high affinity for the desired target are isolated. These cycles consist of a series of steps that include binding to the target, washing to remove unbound sequences, and finally, elution of target-bound aptamers. This process is repeated several times iteratively until the aptamers with high specificity and affinity are selected by Novaptech. The specific binding mechanism of aptamers to their target Aptamers use a variety of molecular interactions to bind to their target hydrogen bonds, electrostatic or\/and stacking interactions, between the aptamer nucleotides and the target residues. This interaction is highly specific, depending on the precise molecular characteristics of the target. Moreover, the aptamer three-dimensional conformation is essential for its specificity. The aptamer adopts a particular folded structure that perfectly matches the shape of the target. This ensures tight, specific binding. Examples of targets commonly studied with aptamers Aptamers have become the tools of choice for the recognition and study of a variety of biomolecular targets. Commonly studied examples include proteins, peptides, viruses, bacteria, cells, drugs and chemical compounds. For example, aptamers have been developed to specifically target proteins such as thrombin, EGFR (epidermal growth factor receptor) and vascular endothelial growth factor (VEGF). These aptamers are used in biomedical research for applications ranging from biomarker to drug analysis. What are the applications of aptamers in research ? Use of aptamers in molecular biology Today, aptamers are essential tools in molecular biology for studying various cellular and molecular processes. They can be used to analyze the structure and function of proteins by binding specifically to them. For example, aptamers are used to study protein-protein interactions, to target specific regions of proteins or to selectively inhibit their activity. These applications are essential for understanding the biological mechanisms underlying many diseases and for developing new therapies. Applications in diagnostics and detection Thanks to their high specificity, aptamers are able to detect specific biomarkers associated with diseases, such as proteins or metabolites. This capability enables Novaptech to create accurate, rapid and sensitive diagnostic tests for diseases such as cancer, viral infections or neurodegenerative diseases. Aptamers are also used to detect toxic substances or contaminants in the environment (pesticides, endocrine disruptors, drug residues\u2026). Aptamers in pharmacology In the pharmaceutical industry, aptamers are playing an increasingly important role in drug discovery. They can be designed to specifically target proteins involved in disease, paving the way for the development of more specific and less toxic drugs. For example, aptamers are being studied to target cell receptors involved in cancer or autoimmune diseases.\u00a0 In addition, aptamers can be used to deliver drugs directly to target cells, thus minimizing undesirable side effects. What are the applications of aptamers in medicine ?<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-1650","page","type-page","status-publish","hentry"],"yoast_head":"\nDefinition of an aptamer | Novaptech - Novaptech<\/title>\n<meta name=\"description\" content=\"In the field of biotechnology, aptamers are attracting growing interest as they play a fundamental role in research and medicine.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/novaptech.com\/en\/definition-of-an-aptamer-novaptech\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Definition of an aptamer | Novaptech - Novaptech\" \/>\n<meta property=\"og:description\" content=\"In the field of biotechnology, aptamers are attracting growing interest as they play a fundamental role in research and medicine.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/novaptech.com\/en\/definition-of-an-aptamer-novaptech\/\" \/>\n<meta property=\"og:site_name\" content=\"Novaptech\" \/>\n<meta property=\"article:modified_time\" content=\"2025-02-28T13:22:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_400_0_0_crop-1.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/novaptech.com\/definition-of-an-aptamer-novaptech\/\",\"url\":\"https:\/\/novaptech.com\/definition-of-an-aptamer-novaptech\/\",\"name\":\"Definition of an aptamer | Novaptech - 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