{"version":"1.0","provider_name":"Novaptech","provider_url":"https:\/\/novaptech.com\/fr","title":"Aptamer-based ELISA like assay - Novaptech","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"sJSYzESUeT\"><a href=\"https:\/\/novaptech.com\/fr\/portfolio_page\/aptamer-based-elisa-like-assay\/\">Test de type ELISA bas\u00e9 sur les aptam\u00e8res<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/novaptech.com\/fr\/portfolio_page\/aptamer-based-elisa-like-assay\/embed\/#?secret=sJSYzESUeT\" width=\"600\" height=\"338\" title=\"\u00ab\u00a0Aptamer-based ELISA like assay\u00a0\u00bb &#8212; Novaptech\" data-secret=\"sJSYzESUeT\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/novaptech.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>","description":"Aptamer-based ELISA like assay Aptamers constitute a good alternative to antibodies in ELISA-like tests (sometime termed ELONA). The test can be performed with two aptamers raised against two different sites of the target molecule. They can also be combined with an antibody. \u00a0Direct, indirect and sandwich configurations can be considered. Novaptech aptaswitch-aptakiss combination is of particular interest for the detection and quantification of small molecules. See for instance as an example of &#8220;sandwich assay&#8221;:\u00a0 Scarano, S., Dausse, E., Crispo, F., Toulm\u00e9, J.-J., &amp; Minunni, M. (2015).\u00a0Design of a dual aptamer-based recognition strategy for human matrix metalloproteinase 9 protein by piezoelectric biosensors.\u00a0Analytica Chimica Acta,\u00a0897."}