{"id":136,"date":"2024-06-02T07:55:47","date_gmt":"2024-06-02T07:55:47","guid":{"rendered":"https:\/\/novaptech.com\/?page_id=136"},"modified":"2025-03-03T16:46:34","modified_gmt":"2025-03-03T16:46:34","slug":"what-are-aptamers-selex","status":"publish","type":"page","link":"https:\/\/novaptech.com\/fr\/what-are-aptamers-selex\/","title":{"rendered":"Aptam\u00e8re | SELEX"},"content":{"rendered":"\t\t
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What are aptamers?<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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About nucleic acid aptamers & in vitro selection<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Aptamer\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Aptamer : a definition<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Aptamers are single-stranded nucleic acids (DNA, RNA or chemically-modified RNA) that fold into complex structures. They are able to bind with high affinity and specificity to a pre-determined target. They can be defined as \u201cartificial antibodies\u201d. Aptamers are selected from a randomly synthesized oligonucleotide library containing about 1015 <\/sup><\/span><\/span>different sequences through an iterative in vitro selection process alternating binding and amplification steps.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tNovaptech custom aptamer services<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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The SELEX method, the selection process used by Novaptech for the identification of \naptamers<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Aptamer<\/span> selection is a biotechnological process we use to identify oligonucleotides (DNA, RNA or chemically-modified RNA) that bind specifically to a given target.
This SELEX method, which stands for Systematic Evolution of Ligands by EXponential enrichment, involves several cycles of aptamer binding to the target, followed by the separation of the complexes formed, amplification of the bound aptamers and their reintroduction into the next selection cycle. Different SELEX strategies are used by Novaptech for the identification of aptamers to small molecules, macromolecules or cells.
This process progressively enriches the aptamer population into molecules that have a high affinity for the intended target. Ultimately, this allows the isolation of aptamers with optimal binding properties for diagnostic, therapeutic, analytical or research applications.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Learn more about aptamers and their advantages in the comparison of aptamers vs antibodies<\/a><\/span>. Delve into the specifics of biomarker analysis<\/a><\/span> and discover the cutting-edge research in our biotechnology laboratory<\/a>,<\/span> along with the innovative techniques in biomarker<\/span> <\/a>analysis.<\/span><\/p><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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The in vitro<\/i><\/b> selection process<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Selection of aptamers is achieved by mixing the target with the library. Novaptech makes use of different proprietary librairies adapted to the target and the final application of the aptamers.
Partitioning between target-bound and free sequences is performed by various methods : filtration, chromatography, centrifugation. Target-bound oligonucleotides are PCR-amplified and the resulting pool is mixed with the target under increased selection pressure. The procedure is repeated several times. Among millions of sequences, the strongest binders will \u00absurvive \u00bb the directed evolution. Aptamers are identified following high throughput sequencing and bioinformatics analysis with Novaptech proprietary algorithms.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tNovaptech custom aptamer services<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Aptamer applications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Aptamers as therapeutics<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Aptamers for diagnostics in human and animal health <\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Aptamers for analytical purposes, health biomarkers, environment and food safety monitoring<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Any question ?
Contact us<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days).<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tContact us<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Biomarker\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Your scientific contact :<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Jean-Jacques Toulm\u00e9, PhD, CSO<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t What is SELEX ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

SELEX is a cutting-edge technique used to identify, from a vast library of oligonucleotide sequences, those with the ability to bind specifically and strongly to a given target, be it a small molecule, a protein or even intact cells.
This process, mastered by Novaptech experts, exploits molecular diversity to isolate aptamers displaying high affinity and specificity for their target.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tHow does the SELEX method work ?<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

The SELEX approach to aptamer selection is a highly complex method that begins with the synthesis of an oligonucleotide library exhibiting a huge diversity, essential for identifying sequences with specific affinity for a given target.
Through a series of successive steps: binding, separation and amplification, this process refines and enriches this library into highly specific aptamers.
This systematic approach ensures efficient and accurate selection, paving the way for innovative applications in various fields of research and therapeutics.<\/p>\n

1. Creating a library : The SELEX method begins with the generation of a library containing a wide variety of oligonucleotide sequences. This diversity is essential for successful selection, as it offers a broad spectrum of potential aptamer candidates.<\/p>\n

2. Target binding : Sequences are brought into contact with the target of interest. Only those that bind to the target are retained for subsequent steps, while non-binders are eliminated.<\/p>\n

3. Separation and recovery : Aptamer-target complexes are separated from non-binding sequences. This stage involves various separation methods such as filtration, the use of affinity columns\/beads or centrifugation.<\/p>\n

4. Amplification : The bound aptamers are then amplified, either by PCR for DNA or by reverse transcription followed by PCR for RNA, to generate the enriched library for an additional selection cycle.<\/p>\n

5. Targeted enrichment : The binding, separation and amplification process is repeated over several cycles to progressively enrich the library with sequences with the highest affinity for the target.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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The SELEX selection principle has revolutionized the development of tools for a multitude of applications, ranging from medical diagnostics and environmental detection to therapeutic targeting.<\/p>\n

Aptamers selected via SELEX by Novaptech offer several advantages over traditional antibodies, including greater stability, less costly synthesis and absence of batch-to-batch variability.<\/p>\n

Applications :<\/strong><\/p>\n

\u00b7 Medical diagnostics : Used in the early detection of diseases through the identification of specific biomarkers.<\/p>\n

\u00b7 Therapeutics : Enable the development of new drugs and\/or the targeting of diseased cells without affecting healthy cells.<\/p>\n

\u00b7 Environmental analysis : Detects specifically contaminants (molecules or microbial organisms) in water, air, and soil.<\/p>\n

\u00b7 And more : actually any application involving antibodies can be translated in and benefit from aptamers<\/p>\n

Advantages over antibodies :<\/strong><\/p>\n

\u00b7 Greater stability : Aptamers are more resistant to temperature and pH variations.<\/p>\n

\u00b7 Cost-effective synthesis : Aptamers are less expensive to produce.<\/p>\n

\u00b7 Reproducibility : Aptamers abolish batch-to-batch variations, ensuring consistent quality.<\/p>\n

\u00b7 Flexibility of modification : Aptamers can be easily modified to improve binding or stability.<\/p>\n

\u00b7 Non-immunogenicity : They are less likely to trigger undesirable immune responses.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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The SELEX process stands out from other ligand selection methods, such as immunoselection or chemical screens, mainly because of its high specificity, speed and low cost.
Unlike immunoselection, which relies on the time-consuming and costly production of antibodies, the SELEX principle enables rapid and efficient selection of aptamers, depending on the nature of the oligonucleotides, without the need for alive biological systems (cells or animals).
As for chemical screening, although broad in its approach, it does not guarantee the high specificity achieved by SELEX.
SELEX offers a more targeted and cost-effective method of identifying molecules with a strong affinity for their target, making this technique particularly attractive for the development of rapid test kits and devices.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\tThe future of SELEX aptamer selection<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

Innovations in SELEX, driven by Novaptech, such as the integration of high-throughput
technologies, have transformed aptamer selection, making the process faster and more accurate.
Our high-throughput SELEX aptamer selection principle uses massive sequencing and screening techniques to analyze millions of sequences simultaneously.
As for future prospects, the ongoing evolution of SELEX, combined with Novaptech’s expertise, promises to open up new avenues in the research and development of innovative treatments.
With the technological advances and research carried out by Novaptech, the SELEX process continues to evolve, integrating high-throughput methods and computational analysis to accelerate aptamer selection and identification. These innovations promise to further broaden the application of aptamers in research.
The SELEX ability to isolate highly specific molecules makes it an essential pillar in the field of biotechnology. With this application in aptamer selection, Novaptech is paving the way for discoveries that will transform our approach to disease detection and treatment.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

What are aptamers? About nucleic acid aptamers & in vitro selection Aptamer : a definition Aptamers are single-stranded nucleic acids (DNA, RNA or chemically-modified RNA) that fold into complex structures. They are able to bind with high affinity and specificity to a pre-determined target. They can be defined as \u201cartificial antibodies\u201d. Aptamers are selected from a randomly synthesized oligonucleotide library containing about 1015 different sequences through an iterative in vitro selection process alternating binding and amplification steps. Novaptech custom aptamer services The SELEX method, the selection process used by Novaptech for the identification of aptamers Aptamer selection is a biotechnological process we use to identify oligonucleotides (DNA, RNA or chemically-modified RNA) that bind specifically to a given target. This SELEX method, which stands for Systematic Evolution of Ligands by EXponential enrichment, involves several cycles of aptamer binding to the target, followed by the separation of the complexes formed, amplification of the bound aptamers and their reintroduction into the next selection cycle. Different SELEX strategies are used by Novaptech for the identification of aptamers to small molecules, macromolecules or cells.This process progressively enriches the aptamer population into molecules that have a high affinity for the intended target. Ultimately, this allows the isolation of aptamers with optimal binding properties for diagnostic, therapeutic, analytical or research applications. Learn more about aptamers and their advantages in the comparison of aptamers vs antibodies. Delve into the specifics of biomarker analysis and discover the cutting-edge research in our biotechnology laboratory, along with the innovative techniques in biomarker analysis. The in vitro selection process Selection of aptamers is achieved by mixing the target with the library. Novaptech makes use of different proprietary librairies adapted to the target and the final application of the aptamers. Partitioning between target-bound and free sequences is performed by various methods : filtration, chromatography, centrifugation. Target-bound oligonucleotides are PCR-amplified and the resulting pool is mixed with the target under increased selection pressure. The procedure is repeated several times. Among millions of sequences, the strongest binders will \u00absurvive \u00bb the directed evolution. Aptamers are identified following high throughput sequencing and bioinformatics analysis with Novaptech proprietary algorithms. Novaptech custom aptamer services Aptamer applications Aptamers as therapeutics Aptamers for diagnostics in human and animal health Aptamers for analytical purposes, health biomarkers, environment and food safety monitoring Any question ? Contact us Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulm\u00e9, PhD, CSO What is SELEX ? SELEX is a cutting-edge technique used to identify, from a vast library of oligonucleotide sequences, those with the ability to bind specifically and strongly to a given target, be it a small molecule, a protein or even intact cells. This process, mastered by Novaptech experts, exploits molecular diversity to isolate aptamers displaying high affinity and specificity for their target. How does the SELEX method work ? The SELEX approach to aptamer selection is a highly complex method that begins with the synthesis of an oligonucleotide library exhibiting a huge diversity, essential for identifying sequences with specific affinity for a given target. Through a series of successive steps: binding, separation and amplification, this process refines and enriches this library into highly specific aptamers. This systematic approach ensures efficient and accurate selection, paving the way for innovative applications in various fields of research and therapeutics. 1. Creating a library : The SELEX method begins with the generation of a library containing a wide variety of oligonucleotide sequences. This diversity is essential for successful selection, as it offers a broad spectrum of potential aptamer candidates. 2. Target binding : Sequences are brought into contact with the target of interest. Only those that bind to the target are retained for subsequent steps, while non-binders are eliminated. 3. Separation and recovery : Aptamer-target complexes are separated from non-binding sequences. This stage involves various separation methods such as filtration, the use of affinity columns\/beads or centrifugation. 4. Amplification : The bound aptamers are then amplified, either by PCR for DNA or by reverse transcription followed by PCR for RNA, to generate the enriched library for an additional selection cycle. 5. Targeted enrichment : The binding, separation and amplification process is repeated over several cycles to progressively enrich the library with sequences with the highest affinity for the target. SELEX applications and benefits The SELEX selection principle has revolutionized the development of tools for a multitude of applications, ranging from medical diagnostics and environmental detection to therapeutic targeting. Aptamers selected via SELEX by Novaptech offer several advantages over traditional antibodies, including greater stability, less costly synthesis and absence of batch-to-batch variability. Applications : \u00b7 Medical diagnostics : Used in the early detection of diseases through the identification of specific biomarkers. \u00b7 Therapeutics : Enable the development of new drugs and\/or the targeting of diseased cells without affecting healthy cells. \u00b7 Environmental analysis : Detects specifically contaminants (molecules or microbial organisms) in water, air, and soil. \u00b7 And more : actually any application involving antibodies can be translated in and benefit from aptamers Advantages over antibodies : \u00b7 Greater stability : Aptamers are more resistant to temperature and pH variations. \u00b7 Cost-effective synthesis : Aptamers are less expensive to produce. \u00b7 Reproducibility : Aptamers abolish batch-to-batch variations, ensuring consistent quality. \u00b7 Flexibility of modification : Aptamers can be easily modified to improve binding or stability. \u00b7 Non-immunogenicity : They are less likely to trigger undesirable immune responses. Why is SELEX the best technology for strong affinity and high specificity ligands ? The SELEX process stands out from other ligand selection methods, such as immunoselection or chemical screens, mainly because of its high specificity, speed and low cost. Unlike immunoselection, which relies on the time-consuming and costly production of antibodies, the SELEX principle enables rapid and<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-136","page","type-page","status-publish","hentry"],"yoast_head":"\nAptamer SELEX - Novaptech<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/novaptech.com\/fr\/what-are-aptamers-selex\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Aptamer SELEX - Novaptech\" \/>\n<meta property=\"og:description\" content=\"What are aptamers? About nucleic acid aptamers & in vitro selection Aptamer : a definition Aptamers are single-stranded nucleic acids (DNA, RNA or chemically-modified RNA) that fold into complex structures. They are able to bind with high affinity and specificity to a pre-determined target. They can be defined as \u201cartificial antibodies\u201d. Aptamers are selected from a randomly synthesized oligonucleotide library containing about 1015 different sequences through an iterative in vitro selection process alternating binding and amplification steps. Novaptech custom aptamer services The SELEX method, the selection process used by Novaptech for the identification of aptamers Aptamer selection is a biotechnological process we use to identify oligonucleotides (DNA, RNA or chemically-modified RNA) that bind specifically to a given target. This SELEX method, which stands for Systematic Evolution of Ligands by EXponential enrichment, involves several cycles of aptamer binding to the target, followed by the separation of the complexes formed, amplification of the bound aptamers and their reintroduction into the next selection cycle. Different SELEX strategies are used by Novaptech for the identification of aptamers to small molecules, macromolecules or cells.This process progressively enriches the aptamer population into molecules that have a high affinity for the intended target. Ultimately, this allows the isolation of aptamers with optimal binding properties for diagnostic, therapeutic, analytical or research applications. Learn more about aptamers and their advantages in the comparison of aptamers vs antibodies. Delve into the specifics of biomarker analysis and discover the cutting-edge research in our biotechnology laboratory, along with the innovative techniques in biomarker analysis. The in vitro selection process Selection of aptamers is achieved by mixing the target with the library. Novaptech makes use of different proprietary librairies adapted to the target and the final application of the aptamers. Partitioning between target-bound and free sequences is performed by various methods : filtration, chromatography, centrifugation. Target-bound oligonucleotides are PCR-amplified and the resulting pool is mixed with the target under increased selection pressure. The procedure is repeated several times. Among millions of sequences, the strongest binders will \u00absurvive \u00bb the directed evolution. Aptamers are identified following high throughput sequencing and bioinformatics analysis with Novaptech proprietary algorithms. Novaptech custom aptamer services Aptamer applications Aptamers as therapeutics Aptamers for diagnostics in human and animal health Aptamers for analytical purposes, health biomarkers, environment and food safety monitoring Any question ? Contact us Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulm\u00e9, PhD, CSO What is SELEX ? SELEX is a cutting-edge technique used to identify, from a vast library of oligonucleotide sequences, those with the ability to bind specifically and strongly to a given target, be it a small molecule, a protein or even intact cells. This process, mastered by Novaptech experts, exploits molecular diversity to isolate aptamers displaying high affinity and specificity for their target. How does the SELEX method work ? The SELEX approach to aptamer selection is a highly complex method that begins with the synthesis of an oligonucleotide library exhibiting a huge diversity, essential for identifying sequences with specific affinity for a given target. Through a series of successive steps: binding, separation and amplification, this process refines and enriches this library into highly specific aptamers. This systematic approach ensures efficient and accurate selection, paving the way for innovative applications in various fields of research and therapeutics. 1. Creating a library : The SELEX method begins with the generation of a library containing a wide variety of oligonucleotide sequences. This diversity is essential for successful selection, as it offers a broad spectrum of potential aptamer candidates. 2. Target binding : Sequences are brought into contact with the target of interest. Only those that bind to the target are retained for subsequent steps, while non-binders are eliminated. 3. Separation and recovery : Aptamer-target complexes are separated from non-binding sequences. This stage involves various separation methods such as filtration, the use of affinity columns\/beads or centrifugation. 4. Amplification : The bound aptamers are then amplified, either by PCR for DNA or by reverse transcription followed by PCR for RNA, to generate the enriched library for an additional selection cycle. 5. Targeted enrichment : The binding, separation and amplification process is repeated over several cycles to progressively enrich the library with sequences with the highest affinity for the target. SELEX applications and benefits The SELEX selection principle has revolutionized the development of tools for a multitude of applications, ranging from medical diagnostics and environmental detection to therapeutic targeting. Aptamers selected via SELEX by Novaptech offer several advantages over traditional antibodies, including greater stability, less costly synthesis and absence of batch-to-batch variability. Applications : \u00b7 Medical diagnostics : Used in the early detection of diseases through the identification of specific biomarkers. \u00b7 Therapeutics : Enable the development of new drugs and\/or the targeting of diseased cells without affecting healthy cells. \u00b7 Environmental analysis : Detects specifically contaminants (molecules or microbial organisms) in water, air, and soil. \u00b7 And more : actually any application involving antibodies can be translated in and benefit from aptamers Advantages over antibodies : \u00b7 Greater stability : Aptamers are more resistant to temperature and pH variations. \u00b7 Cost-effective synthesis : Aptamers are less expensive to produce. \u00b7 Reproducibility : Aptamers abolish batch-to-batch variations, ensuring consistent quality. \u00b7 Flexibility of modification : Aptamers can be easily modified to improve binding or stability. \u00b7 Non-immunogenicity : They are less likely to trigger undesirable immune responses. Why is SELEX the best technology for strong affinity and high specificity ligands ? The SELEX process stands out from other ligand selection methods, such as immunoselection or chemical screens, mainly because of its high specificity, speed and low cost. Unlike immunoselection, which relies on the time-consuming and costly production of antibodies, the SELEX principle enables rapid and\" \/>\n<meta property=\"og:url\" content=\"https:\/\/novaptech.com\/fr\/what-are-aptamers-selex\/\" \/>\n<meta property=\"og:site_name\" content=\"Novaptech\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-03T16:46:34+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/\",\"url\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/\",\"name\":\"Aptamer SELEX - Novaptech\",\"isPartOf\":{\"@id\":\"https:\/\/novaptech.com\/en\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg\",\"datePublished\":\"2024-06-02T07:55:47+00:00\",\"dateModified\":\"2025-03-03T16:46:34+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/novaptech.com\/what-are-aptamers-selex\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-FR\",\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage\",\"url\":\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg\",\"contentUrl\":\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/novaptech.com\/what-are-aptamers-selex\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/novaptech.com\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Aptamer SELEX\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/novaptech.com\/en\/#website\",\"url\":\"https:\/\/novaptech.com\/en\/\",\"name\":\"Novaptech\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/novaptech.com\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/novaptech.com\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-FR\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/novaptech.com\/en\/#organization\",\"name\":\"Novaptech\",\"url\":\"https:\/\/novaptech.com\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-FR\",\"@id\":\"https:\/\/novaptech.com\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/06\/cropped-LOGO.png\",\"contentUrl\":\"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/06\/cropped-LOGO.png\",\"width\":335,\"height\":140,\"caption\":\"Novaptech\"},\"image\":{\"@id\":\"https:\/\/novaptech.com\/en\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Aptamer SELEX - Novaptech","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/novaptech.com\/fr\/what-are-aptamers-selex\/","og_locale":"fr_FR","og_type":"article","og_title":"Aptamer SELEX - Novaptech","og_description":"What are aptamers? About nucleic acid aptamers & in vitro selection Aptamer : a definition Aptamers are single-stranded nucleic acids (DNA, RNA or chemically-modified RNA) that fold into complex structures. They are able to bind with high affinity and specificity to a pre-determined target. They can be defined as \u201cartificial antibodies\u201d. Aptamers are selected from a randomly synthesized oligonucleotide library containing about 1015 different sequences through an iterative in vitro selection process alternating binding and amplification steps. Novaptech custom aptamer services The SELEX method, the selection process used by Novaptech for the identification of aptamers Aptamer selection is a biotechnological process we use to identify oligonucleotides (DNA, RNA or chemically-modified RNA) that bind specifically to a given target. This SELEX method, which stands for Systematic Evolution of Ligands by EXponential enrichment, involves several cycles of aptamer binding to the target, followed by the separation of the complexes formed, amplification of the bound aptamers and their reintroduction into the next selection cycle. Different SELEX strategies are used by Novaptech for the identification of aptamers to small molecules, macromolecules or cells.This process progressively enriches the aptamer population into molecules that have a high affinity for the intended target. Ultimately, this allows the isolation of aptamers with optimal binding properties for diagnostic, therapeutic, analytical or research applications. Learn more about aptamers and their advantages in the comparison of aptamers vs antibodies. Delve into the specifics of biomarker analysis and discover the cutting-edge research in our biotechnology laboratory, along with the innovative techniques in biomarker analysis. The in vitro selection process Selection of aptamers is achieved by mixing the target with the library. Novaptech makes use of different proprietary librairies adapted to the target and the final application of the aptamers. Partitioning between target-bound and free sequences is performed by various methods : filtration, chromatography, centrifugation. Target-bound oligonucleotides are PCR-amplified and the resulting pool is mixed with the target under increased selection pressure. The procedure is repeated several times. Among millions of sequences, the strongest binders will \u00absurvive \u00bb the directed evolution. Aptamers are identified following high throughput sequencing and bioinformatics analysis with Novaptech proprietary algorithms. Novaptech custom aptamer services Aptamer applications Aptamers as therapeutics Aptamers for diagnostics in human and animal health Aptamers for analytical purposes, health biomarkers, environment and food safety monitoring Any question ? Contact us Any question about selection service or assay development ? Please fill in the form below with relevant details for helping us providing an informative answer. Telephone or video call can be arranged. NDA can be signed, would you wish so. We will answer you as quickly as possible (generally less than 2 working days). Contact us Your scientific contact : Jean-Jacques Toulm\u00e9, PhD, CSO What is SELEX ? SELEX is a cutting-edge technique used to identify, from a vast library of oligonucleotide sequences, those with the ability to bind specifically and strongly to a given target, be it a small molecule, a protein or even intact cells. This process, mastered by Novaptech experts, exploits molecular diversity to isolate aptamers displaying high affinity and specificity for their target. How does the SELEX method work ? The SELEX approach to aptamer selection is a highly complex method that begins with the synthesis of an oligonucleotide library exhibiting a huge diversity, essential for identifying sequences with specific affinity for a given target. Through a series of successive steps: binding, separation and amplification, this process refines and enriches this library into highly specific aptamers. This systematic approach ensures efficient and accurate selection, paving the way for innovative applications in various fields of research and therapeutics. 1. Creating a library : The SELEX method begins with the generation of a library containing a wide variety of oligonucleotide sequences. This diversity is essential for successful selection, as it offers a broad spectrum of potential aptamer candidates. 2. Target binding : Sequences are brought into contact with the target of interest. Only those that bind to the target are retained for subsequent steps, while non-binders are eliminated. 3. Separation and recovery : Aptamer-target complexes are separated from non-binding sequences. This stage involves various separation methods such as filtration, the use of affinity columns\/beads or centrifugation. 4. Amplification : The bound aptamers are then amplified, either by PCR for DNA or by reverse transcription followed by PCR for RNA, to generate the enriched library for an additional selection cycle. 5. Targeted enrichment : The binding, separation and amplification process is repeated over several cycles to progressively enrich the library with sequences with the highest affinity for the target. SELEX applications and benefits The SELEX selection principle has revolutionized the development of tools for a multitude of applications, ranging from medical diagnostics and environmental detection to therapeutic targeting. Aptamers selected via SELEX by Novaptech offer several advantages over traditional antibodies, including greater stability, less costly synthesis and absence of batch-to-batch variability. Applications : \u00b7 Medical diagnostics : Used in the early detection of diseases through the identification of specific biomarkers. \u00b7 Therapeutics : Enable the development of new drugs and\/or the targeting of diseased cells without affecting healthy cells. \u00b7 Environmental analysis : Detects specifically contaminants (molecules or microbial organisms) in water, air, and soil. \u00b7 And more : actually any application involving antibodies can be translated in and benefit from aptamers Advantages over antibodies : \u00b7 Greater stability : Aptamers are more resistant to temperature and pH variations. \u00b7 Cost-effective synthesis : Aptamers are less expensive to produce. \u00b7 Reproducibility : Aptamers abolish batch-to-batch variations, ensuring consistent quality. \u00b7 Flexibility of modification : Aptamers can be easily modified to improve binding or stability. \u00b7 Non-immunogenicity : They are less likely to trigger undesirable immune responses. Why is SELEX the best technology for strong affinity and high specificity ligands ? The SELEX process stands out from other ligand selection methods, such as immunoselection or chemical screens, mainly because of its high specificity, speed and low cost. Unlike immunoselection, which relies on the time-consuming and costly production of antibodies, the SELEX principle enables rapid and","og_url":"https:\/\/novaptech.com\/fr\/what-are-aptamers-selex\/","og_site_name":"Novaptech","article_modified_time":"2025-03-03T16:46:34+00:00","og_image":[{"url":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg","type":"","width":"","height":""}],"twitter_card":"summary_large_image","twitter_misc":{"Dur\u00e9e de lecture estim\u00e9e":"7 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/","url":"https:\/\/novaptech.com\/what-are-aptamers-selex\/","name":"Aptamer SELEX - Novaptech","isPartOf":{"@id":"https:\/\/novaptech.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage"},"image":{"@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage"},"thumbnailUrl":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg","datePublished":"2024-06-02T07:55:47+00:00","dateModified":"2025-03-03T16:46:34+00:00","breadcrumb":{"@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/#breadcrumb"},"inLanguage":"fr-FR","potentialAction":[{"@type":"ReadAction","target":["https:\/\/novaptech.com\/what-are-aptamers-selex\/"]}]},{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/#primaryimage","url":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg","contentUrl":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/05\/thumb_600_0_0_0_auto-1.jpg"},{"@type":"BreadcrumbList","@id":"https:\/\/novaptech.com\/what-are-aptamers-selex\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/novaptech.com\/en\/"},{"@type":"ListItem","position":2,"name":"Aptamer SELEX"}]},{"@type":"WebSite","@id":"https:\/\/novaptech.com\/en\/#website","url":"https:\/\/novaptech.com\/en\/","name":"Novaptech","description":"","publisher":{"@id":"https:\/\/novaptech.com\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/novaptech.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-FR"},{"@type":"Organization","@id":"https:\/\/novaptech.com\/en\/#organization","name":"Novaptech","url":"https:\/\/novaptech.com\/en\/","logo":{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/novaptech.com\/en\/#\/schema\/logo\/image\/","url":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/06\/cropped-LOGO.png","contentUrl":"https:\/\/novaptech.com\/wp-content\/uploads\/2024\/06\/cropped-LOGO.png","width":335,"height":140,"caption":"Novaptech"},"image":{"@id":"https:\/\/novaptech.com\/en\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/pages\/136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/comments?post=136"}],"version-history":[{"count":156,"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/pages\/136\/revisions"}],"predecessor-version":[{"id":4609,"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/pages\/136\/revisions\/4609"}],"wp:attachment":[{"href":"https:\/\/novaptech.com\/fr\/wp-json\/wp\/v2\/media?parent=136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}