{"id":1395,"date":"2024-06-07T02:51:55","date_gmt":"2024-06-07T02:51:55","guid":{"rendered":"https:\/\/novaptech.com\/?page_id=1395"},"modified":"2025-02-11T14:13:54","modified_gmt":"2025-02-11T14:13:54","slug":"aptamer-based-elisa-like-assay","status":"publish","type":"page","link":"https:\/\/novaptech.com\/fr\/portfolio_page\/aptamer-based-elisa-like-assay\/","title":{"rendered":"Test de type ELISA bas\u00e9 sur les aptam\u00e8res"},"content":{"rendered":"
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Test de type ELISA bas\u00e9 sur les aptam\u00e8res<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Les aptam\u00e8res constituent une bonne alternative aux anticorps dans les tests de type ELISA (parfois appel\u00e9s ELONA). Le test peut \u00eatre r\u00e9alis\u00e9 avec deux aptam\u00e8res dirig\u00e9s contre deux sites diff\u00e9rents de la mol\u00e9cule cible. Ils peuvent \u00e9galement \u00eatre associ\u00e9s \u00e0 un anticorps. \u00a0Des configurations directes, indirectes et sandwich peuvent \u00eatre envisag\u00e9es. La combinaison aptaswitch-aptakiss de Novaptech pr\u00e9sente un int\u00e9r\u00eat particulier pour la d\u00e9tection et la quantification de petites mol\u00e9cules.<\/p>

Exemple d'application \u00ab sandwich assay \u00bb\u00a0<\/span><\/p>

Scarano, S., Dausse, E., Crispo, F., Toulm\u00e9, J.-J., & Minunni, M. (2015).\u00a0<\/span>Design of a dual aptamer-based recognition strategy for human matrix metalloproteinase 9 protein by piezoelectric biosensors.<\/strong><\/a>\u00a0<\/span>Analytica Chimica Acta<\/em>,\u00a0<\/span>897.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Aptamer-based ELISA like assay Aptamers constitute a good alternative to antibodies in ELISA-like tests (sometime termed ELONA). The test can be performed with two aptamers raised against two different sites of the target molecule. They can also be combined with an antibody. \u00a0Direct, indirect and sandwich configurations can be considered. Novaptech aptaswitch-aptakiss combination is of particular interest for the detection and quantification of small molecules. See for instance as an example of “sandwich assay”:\u00a0 Scarano, S., Dausse, E., Crispo, F., Toulm\u00e9, J.-J., & Minunni, M. (2015).\u00a0Design of a dual aptamer-based recognition strategy for human matrix metalloproteinase 9 protein by piezoelectric biosensors.\u00a0Analytica Chimica Acta,\u00a0897.<\/p>","protected":false},"author":1,"featured_media":0,"parent":1339,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-1395","page","type-page","status-publish","hentry"],"yoast_head":"\nAptamer-based ELISA like assay - Novaptech<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/novaptech.com\/fr\/portfolio_page\/aptamer-based-elisa-like-assay\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Aptamer-based ELISA like assay - Novaptech\" \/>\n<meta property=\"og:description\" content=\"Aptamer-based ELISA like assay Aptamers constitute a good alternative to antibodies in ELISA-like tests (sometime termed ELONA). The test can be performed with two aptamers raised against two different sites of the target molecule. They can also be combined with an antibody. \u00a0Direct, indirect and sandwich configurations can be considered. Novaptech aptaswitch-aptakiss combination is of particular interest for the detection and quantification of small molecules. See for instance as an example of “sandwich assay”:\u00a0 Scarano, S., Dausse, E., Crispo, F., Toulm\u00e9, J.-J., & Minunni, M. 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